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A step by step tutorial on quantification of agarose gel bands using ImageJ software. Go to Analyze --> Gels --> Select first lane (then use ctrl + 2 to select all the other lanes and ctrl + 3 to plot the data). This automatically Quantifying Western Blot images using ImageJ | Densitometric analysis In this video I'm going to be comparing our Phoretix 1D Western blot/electrophoresis gel image analysis software to the academic Learn how to normalize your western blot signal to your total protein using TotalLab's Phoretix 1D image analysis software. ...